This involves chemicals (acid tyrodes) used to produce a weakened area of the zona. The chemical is applied through a microtool, manipulated by an embryologist. It is not entirely accurate, as when working with such small amounts of chemicals, it is hard to quantify the dose with pinpoint accuracy. The acid then has to be washed off in order to prevent further damage to the embryo
LASER assisted hatching:
The zona is drilled by the microlaser system called Fertilase. It creates a clean-cut precise incision in the zona.
This is more accurate for several reasons:
The process is fast compared to the chemical method, thus the embryo does not have to be held still by a suction pipette. As well as this factor, we use a vibration free table to avoid any accidental movement of the embryo.
The accuracy of the laser is predetermined by its programming, and is therefore not dependent on the skill of the operator. There is a high degree of reproducible accuracy.